Cluster Facilities
Genomics including PCR, RT-PCR, Arrays & Sequencing
Real Time PCR - Applied Biosystems PCR Fast 7500
Real Time PCR - Applied Biosystems PCR Fast 7500| Where | IRC, Level 2 Daly Wing |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Contact Lab for detailed training and usage instructions |
| Documentation | PCRfast_cms_040314.pdf |  |
Real Time PCR
Real Time PCR - Real time PCR ABI Prism 7000| Where | Clinical Sciences Building |
|---|---|
| Contact | Alison Cox, 2583
ajcox@medstv.unimelb.edu.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Training Required | |
Real Time PCR
Real Time PCR - Real time PCR ABI| Where | SVI |
|---|---|
| Contact | Narelle McGregor, 2480
nmcgregor@svi.edu.au |
| Current Level of Use | >8 Hours per day |
| Usage restrictions | Training Required, provide own consumables | |
Nano Drop Spectrophotometer
Nano Drop Spectrophotometer ND1000| Where | IRC, Level 2 Daly Wing | |
|---|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au | |
| Current Level of Use | Few times per week | |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
| Documnetation | nd-1000-v3.7-users-manual.pdf | |
Roche LightCycler
Roche LightCycler 480 II: This machine can be used for real-time PCR to quantitate gene expression and perform high-throughput genotyping of different types of sequence variants.| Where | SVI |
|---|---|
| Contact | Tom Brodnicki, 2502
tbrodnicki@svi.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | Restricted access room, training required | |
Gene Navigator Pulsed field gel
Gene Navigator Pulsed field gel electrophoresis system - separation of very large pieces of DNA| Where | SVI |
|---|---|
| Contact | Jörg Heierhost, 2503
jheierhost@svi.edu.au |
| Current Level of Use | Less than weekly |
| Usage restrictions | Training required, Supervision required during use | |
Agilent 2100 Bioanalyser
Agilent 2100 Bioanalyser - measure quality & quantity of RNA| Where | Clinical Sciences Building |
|---|---|
| Contact | Alison Cox, 2583
ajcox@medstv.unimelb.edu.au |
| Current Level of Use | Less than weekly |
| Usage restrictions | Training required, own reagents to be supplied (expensive) | |
Cell sorting, other cell-related applications (incl haematology)
Microelectrode Array
Microelectrode Array (Multichannel system) By utilising multiple (60) electrodes embedded in a glass culture dish, the system allow simultaneous targeting of several sites for stimulation and recording of cellular electrical activity. The system comes with a filter amplifier, software for data acquisition and analysis.| Where | O'Brian Institute |
|---|---|
| Contact | Nao Kobayashi, 4030
naok@unimelb.edu.au |
| Current Level of Use | Few times per week |
| Usage restrictions | None | |
Rotorgene
Corbett Research Rotorgene 3000| Where | SVI |
|---|---|
| Contact | Lina Mariana, 3513
lmariana@svi.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | None | |
Olympus Live Cell Imager
Olympus Live Cell Imager - cell culture fluoresence microscope| Where | SVI |
|---|---|
| Contact | Mark Waltham, 2566
mwaltham@svi.edu.au |
| Steve Tonna, 3285
stonna@svi.edu.au | |
| Current Level of Use | Few times a week |
| Usage restrictions | Training required | |
Haematological analyzer
Haematological analyzer (Sysmex K21N)| Where | SVI |
|---|---|
| Contact | Julie Quach, 2480
jquach@svi.edu.au |
| Current Level of Use | Few times a week |
| Usage restrictions | None | |
Refrigerated Superspeed centrifuge
Sorvall RC6 Refrigerated Superspeed centrifuge. Rotors: carbon fiber-6x250y, 8x50y & 6x500y.| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Contact Lab for detailed training and usage instructions |
| Documentation | SORVALL_RC-6_Plus.pdf | |
Whole Blood & Platelet Aggregometer
Chrono-log Whole blood aggregometer: Model 570-VS. This model is capable of both whole blood and platelet aggregometry.| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Training required | |
Amelung Coagulometer
KC4 Amelung Coagulometer ‐ Trinity Biotech 40549| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
Platelet Aggregometer (verify now)
Platelet Aggregometer (verify now)| Where | Cardiac Cath Lab |
|---|---|
| Contact | Rob Whitbourn, 4439
robert.whitbourn@svhm.org.au |
| Current Level of Use | Weekly |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
ACT machine (Activated Clotting Time)
ACT machine (Activated Clotting Time)| Where | Cardiac Cath Lab |
|---|---|
| Contact | Rob Whitbourn, 4439
robert.whitbourn@svhm.org.au |
| Current Level of Use | |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
Shandon Cytospin 4
ABI Q-TAF Mass Spectrometer.| Where | SVI |
|---|---|
| Contact | Kate Graham, 2480
kgraham@svi.edu.au |
| Current Level of Use | Weekly |
| Usage restrictions | None | |
Cell separation centrifuge
Cobe 2991 cell separation centrifuge with refrigeration. Used for human islet cell isolation.| Where | SVI |
|---|---|
| Contact | Tom Loudovaris, 2503
tloudovaris@svi.edu.au |
| Current Level of Use | Less than weekly |
| Usage restrictions | Training required | |
Tissue culture incubator
Tissue culture incubator set at 22C used for culture of human islets.| Where | SVI |
|---|---|
| Contact | Tom Loudovaris, 2503
tloudovaris@svi.edu.au |
| Current Level of Use | Weekly |
| Usage restrictions | Training required | |
Flow cytometry facility
Flow cytometry facility FACS Calibur, FACS Aria, Fortessa. Details on SVI website.| Where | SVI |
|---|---|
| Contact | Natalie Sanders, 3511
nsanders@svi.edu.au |
| Current Level of Use | 1‐4 hours/day |
| Usage restrictions | Training required, Dedicated operator, Charges apply | |
Vascular Imaging
ILab™ Intravascular Ultrasound (IVUS)
ILab™ Intravascular Ultrasound (IVUS)| Where | Cardiac Cath Lab |
|---|---|
| Contact | Rob Whitbourn, 4439
robert.whitbourn@svhm.org.au |
| Current Level of Use | |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
Volcano™ Intravascular Ultrasound (IVUS)
Volcano™ Intravascular Ultrasound (IVUS)| Where | Cardiac Cath Lab |
|---|---|
| Contact | Rob Whitbourn, 4439
robert.whitbourn@svhm.org.au |
| Current Level of Use | |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
Litelab™ Optical Coherance Tanography machine (OCT)
Litelab™ Optical Coherance Tanography (OCT)machine| Where | Cardiac Cath Lab |
|---|---|
| Contact | Rob Whitbourn, 4439
robert.whitbourn@svhm.org.au |
| Current Level of Use | |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
RADI – Analyser – Pressure reading guidewire system
RADI – Analyser – Pressure reading guidewire system| Where | Cardiac Cath Lab |
|---|---|
| Contact | Rob Whitbourn, 4439
robert.whitbourn@svhm.org.au |
| Current Level of Use | |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
Echocardiography / Transoesophageal Oesophageal Echo
Echocardiography / Transoesophageal Oesophageal Echo| Where | Cardiac Cath Lab |
|---|---|
| Contact | Rob Whitbourn, 4439
robert.whitbourn@svhm.org.au |
| Current Level of Use | |
| Usage restrictions | Contact Lab for detailed training and usage instructions | |
Proteomics including Mass Spectrometer
Mass Spectrometer
ABI Q-TAF Mass Spectrometer| Where | SVI |
|---|---|
| Contact | Bruce Kemp, 2480
bkemp@svi.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | Dedicated Operator | |
Microscopy, Imaging, Histology, in vivo (animal) work
BSBL X-ray Facility
BSBL X-ray Facility (2 high power X-ray generators, imaging plate detectors, linux computer network, circular dichroism spectrometer, dynamic light scattering instrument, liquid handling robots, electrophysiology rig)| Where | SVI |
|---|---|
| Contact | Belinda Michell, 2480
bmichell@svi.edu.au |
| Current Level of Use | >8 hours per day |
| Usage restrictions | Restricted access room, dedicated Operator | |
Leica 2165 Microtome
Leica 2165 automated microtome with Tungsten Carbide blades for plastic sectioning| Where | SVI |
|---|---|
| Contact | Ingrid Poulton, 2511
lpoulton@svi.edu.au |
| Current Level of Use | 1-4 hours per day |
| Usage restrictions | Restricted access room, dedicated Operator, business hours only | |
Leica Rotary Microtome
Leica Rotary Microtome| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Training required | |
Micro CT
Skyscan 1076 in vivo micro computer tomography scanner| Where | Bioresources Centre |
|---|---|
| Contact | Nicole Walsh, 2510
nwalsh@svi.edu.au |
| Current Level of Use | >8 hours per day |
| Usage restrictions | Restricted access room, training required, maintenance fee applies | |
Zeiss Axiovert Inverted Microscope
Zeiss Axiovert 25 inverted microscope with Zeiss Axiovision Camera. long-working distance 10-40x, 100X oil regular immunofluorescence, bright field or phase contrast microscopy on fixed or live cells.| Where | SVI |
|---|---|
| Contact | Jörg Heierhorst, 2503
jheierhorst@svi.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | Restricted access room, training required | |
Aperio ScanScope
Aperio ScanScope model CS| Where | DOM - CSB |
|---|---|
| Contact | Michael Zhang, 2256
yz@medstv.unimelb.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | Training required | |
Osteomeasure Image Analysis system
Osteomeasure Image Analysis system| Where | SVI |
|---|---|
| Contact | Natalie Sims, 2555
nsims@svi.edu.au |
| Current Level of Use | >6 Hours per day |
| Usage restrictions | training required | |
Leitz Cryostat
Leitz Cryostat 1720| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Training required | |
Olympus Provis Microscope
Olympus Provis Microscope (UV/Fluorescent/Brightfield) + Spot Camera and image software| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Training required, supervision required during use. Contact Lab for detailed training and usage instructions | |
BMG Fluostar Galaxy
BMG Fluostar Galaxy fully automated microplate based multi-detection| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Training required | |
Zeiss Axiovert 25 Inverted Microscope
Zeiss Axiovert 25 Inverted Microscope with Zeiss Axiovision Camera| Where | IRC |
|---|---|
| Contact | Neil Wilson, 3146
neil.wilson@svhm.org.au |
| Current Level of Use | Few times per week |
| Usage restrictions | Training required | |
Phase contrast microscope
Phase contrast microscope fitted with digital camera Olympus CKX41| Where | SVI |
|---|---|
| Contact | Jan Allison, 2756
jallison@svi.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | None | |
Fluorescent microscope
Fluorescent microscope fitted with digital camera Leitz Laborlux FITC and Texas Red filters| Where | SVI |
|---|---|
| Contact | Jan Allison, 2756
jallison@svi.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | None | |
Zeiss AxioVision 4 image analysis
Zeiss AxioVision 4 image analysis| Where | DOM - CSB |
|---|---|
| Contact | Michael Zhang, 2256
yz@medstv.unimelb.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | Training required | |
Cobas Integra autoanalyzer
Cobas Integra autoanalyzer| Where | DOM - Daly L4 |
|---|---|
| Contact | Andrzej Januszewski, 2664
janand@medstv.unimelb.edu.au |
| Current Level of Use | 1-4 Hours per day |
| Usage restrictions | Training required. Users need to provide ALL consumables BEFORE commencing the training/usage of the analyzer | |
Confocal Microscope
Confocal Microscope| Where | DOM - CSB |
|---|---|
| Contact | Michael Zhang, 2256
yz@medstv.unimelb.edu.au |
| Current Level of Use | Less than weekly |
| Usage restrictions | Training required | |
Cryostat
Cryostat| Where | DOM - CSB |
|---|---|
| Contact | Michael Zhang, 2256
yz@medstv.unimelb.edu.au |
| Current Level of Use | Less than weekly |
| Usage restrictions | Training required, University restricted equipment | |
Echocardiogram
Echocardiogram| Where | BRC |
|---|---|
| Contact | Michael Zhang, 2256
yz@medstv.unimelb.edu.au |
| Current Level of Use | Less than weekly |
| Usage restrictions | Training required, Restricted access room | |
Millar cath
Millar catheter for in vivo heart haemodynamic functional studies| Where | BRC |
|---|---|
| Contact | Michael Zhang, 2256
yz@medstv.unimelb.edu.au |
| Current Level of Use | Less than weekly |
| Usage restrictions | Training required, Restricted access room | |
Offsite Facilities
MDHS Research Domains
A listing of facilities, resources and services that are available, by negotiation with the named contacts, to members of the research community of the faculty encompassing all its departments and schools.| Where | www.mdhs.unimelb.edu.au/research_domains/enabling_technologies | |
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Victorian Platform Technologies Network
The VPTN is a network of biomedical research facilities that provide state-of-the-art infrastructure and expertise to the biomedical, health and biotech research sector in Victoria, throughout Australia and around the world.| Where | www.platformtechnologies.org | |
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